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NOD1 Polyclonal antibody

NOD1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 14770-1-AP

Print datasheet

Synonyms

CARD4, CLR7.1, NLRC1, NOD1

验证数据展示

  • WB analysis using 14770-1-AP

    WB analysis using 14770-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14770-1-AP (NOD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected in4T1 cells, RAW 264.7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14770-1-AP targets NOD1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen NOD1 fusion protein Ag6567 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nucleotide-binding oligomerization domain containing 1
Synonyms CARD4, CLR7.1, NLRC1, NOD1
Calculated Molecular Weight 108 kDa
Observed Molecular Weight90-100 kDa
GenBank Accession NumberBC040339
Gene Symbol NOD1
Gene ID (NCBI) 10392
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y239
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NOD1 protein contains a caspase recruitment domain (CARD). NOD1 is a member of NOD-like receptor protein family and is a close relative of NOD2. NOD1 is ubiquitously expressed and recognizes bacterial peptidoglycan fragments, including d-glutamyl-meso-diaminopimelic acid found in gram-negative and some gram-positive bacteria. NOD1 plays a role in innate immunity by acting as a pattern-recognition receptor (PRR) that binds bacterial peptidoglycans and initiates inflammation. This protein has also been implicated in the immune response to viral and parasitic infection.

实验方案

Product Specific Protocols
WB protocol for NOD1 antibody 14770-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols