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Noggin Polyclonal antibody

Noggin Polyclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 30023-1-AP

Print datasheet

Synonyms

NOG, SYNS1, SYM1

验证数据展示

  • WB analysis using 30023-1-AP

    WB analysis using 30023-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30023-1-AP (NOG antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • FC experiment of PC-3 using 30023-1-AP

    FC experiment of PC-3 using 30023-1-AP

    1x10^6 PC-3 cells were intracellularly stained with 0.4 ug Noggin Polyclonal antibody (30023-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive FC (Intra) detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

30023-1-AP targets Noggin in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen Noggin fusion protein Ag32755 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name noggin
Synonyms NOG, SYNS1, SYM1
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 32 kDa, 64 kDa
GenBank Accession NumberBC034027
Gene Symbol Noggin
Gene ID (NCBI) 9241
RRIDAB_2935501
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13253
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Noggin is an extracellular polypeptide acting as an antagonist of bone morphogenetic proteins (BMPs) regulating embryonal development. Noggin inhibits activity of BMP-2, -4, -7, -13, and -14. Noggin is present extracellularly in the matrix or retained at the cell surface via interaction with heparin sulfate proteoglycans. In early development stages, Noggin is produced by the Spemann organizer, allowing dorsal-ventral patterning of BMPs (PMID: 8752214). Subsequently, Noggin is expressed by the notochord regulating BMP-4 signaling in neurogenesis. Additionally, Noggin is present during development in the dermal papilla, connective tissue of the hair follicle, lens, retina, and periocular mesenchyme, as well as in the mesoderm lineage regulating development of the bone, cartilage, and muscles.

实验方案

Product Specific Protocols
WB protocol for Noggin antibody 30023-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Nanobiotechnology

Exosomes derived from mouse vibrissa dermal papilla cells promote hair follicle regeneration during wound healing by activating Wnt/β-catenin signaling pathway

Authors - Yage Shang
View Article