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验证数据展示

  • WB analysis using 12043-1-AP

    WB analysis using 12043-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12043-1-AP (NOL8 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IP experiment of COLO 320 using 12043-1-AP

    IP experiment of COLO 320 using 12043-1-AP

    IP result of anti-NOL8 (IP:12043-1-AP, 4ug; Detection:12043-1-AP 1:4000) with COLO 320 cells lysate 2560 ug.

NOL8 Polyclonal antibody

NOL8 Polyclonal Antibody for WB, IP, ELISA
Cat No. 12043-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

NOP132, NOL 8, C9orf34

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells, Jurkat cells
Positive IP detected inCOLO 320 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12043-1-AP targets NOL8 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NOL8 fusion protein Ag2672 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nucleolar protein 8
别名 NOP132, NOL 8, C9orf34
计算分子量 132 kDa
观测分子量 150 kDa
GenBank蛋白编号BC013788
基因名称 NOL8
Gene ID (NCBI) 55035
RRIDAB_2152001
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ76FK4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NOL8 (Nucleolar Protein 8) plays an essential role in the survival of diffuse-type gastric cancer cells,and knocking down NOL8 promoted cell apoptosis. The structure analysis suggested that NOL8 contains a RNA binding motif, which was consistent with the study that NOL8 interacted with DEAD-box RNA helicase

实验方案

Product Specific Protocols
WB protocol for NOL8 antibody 12043-1-APDownload protocol
IP protocol for NOL8 antibody 12043-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols