验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 1 publications below |
产品信息
16083-1-AP targets NOL9 in WB, IF, ELISA, FRAP applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF, FRAP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | NOL9 fusion protein Ag9082 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nucleolar protein 9 |
| 别名 | NOL9, nucleolar protein 9 |
| 计算分子量 | 702 aa, 79 kDa |
| 观测分子量 | 79 kDa |
| GenBank蛋白编号 | BC009257 |
| 基因名称 | NOL9 |
| Gene ID (NCBI) | 79707 |
| RRID | AB_11124314 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5SY16 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NOL9, also named as Polynucleotide 5'-hydroxyl-kinase NOL9, is a 702 amino acid protein, which belongs to the Clp1 family. NOL9 localizes in the nucleus and as a polynucleotide 5'-kinase is involved in rRNA processing. its polynucleotide kinase activity is required for efficient generation of the 5.8S and 28S rRNAs from the 32S precursor.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NOL9 antibody 16083-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Early rRNA processing is a stress-dependent regulatory event whose inhibition maintains nucleolar integrity. | ||
Mol Biol Cell LAS1L interacts with the mammalian Rix1 complex to regulate ribosome biogenesis. | ||
Cancer Res Commun USP36 SUMOylates Las1L and promotes its function in pre-rRNA ITS2 processing |
