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验证数据展示

  • WB analysis of mouse colon using 17792-1-AP

    WB analysis of mouse colon using 17792-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 17792-1-AP (NOMO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 17792-1-AP

    IHC staining of human colon using 17792-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 17792-1-AP (NOMO1 Antibody) at dilution of 1:50.

  • IF Staining of MCF-7 using 17792-1-AP

    IF Staining of MCF-7 using 17792-1-AP

    Immunofluorescent analysis of MCF-7 cells, using NOMO1 antibody 17792-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of human colon cancer using 17792-1-AP

    IHC staining of human colon cancer using 17792-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17792-1-AP (NOMO1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 17792-1-AP

    IHC staining of human colon using 17792-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 17792-1-AP (NOMO1 Antibody) at dilution of 1:50.

  • IP experiment of A431 using 17792-1-AP

    IP experiment of A431 using 17792-1-AP

    IP result of anti-NOMO1 (IP:17792-1-AP, 4ug; Detection:17792-1-AP 1:1000) with A431 cells lysate 1520ug.

NOMO1 Polyclonal antibody

NOMO1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 17792-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

pM5 protein, PM5, Nomo, Nodal modulator 1, BOS complex subunit NOMO1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse colon tissue, A431 cells, COLO 320 cells, human brain tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

17792-1-AP targets NOMO1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原 NOMO1 fusion protein Ag11895 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NODAL modulator 1
别名 pM5 protein, PM5, Nomo, Nodal modulator 1, BOS complex subunit NOMO1
计算分子量 1222 aa, 134 kDa
观测分子量 135 kDa
GenBank蛋白编号BC065535
基因名称 NOMO1
Gene ID (NCBI) 23420
RRIDAB_2152031
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15155
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NOMO1 antibody 17792-1-APDownload protocol
IHC protocol for NOMO1 antibody 17792-1-APDownload protocol
IF protocol for NOMO1 antibody 17792-1-APDownload protocol
IP protocol for NOMO1 antibody 17792-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Clin Sci (Lond)

DLX3 promotes bone marrow mesenchymal stem cell proliferation through H19/miR-675 axis.

Authors - Na Zhao
View Article