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NOMO2 Polyclonal antibody

NOMO2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat No : 14328-1-AP

Print datasheet

Synonyms

pM5 protein 2, Nodal modulator 2, BOS complex subunit NOMO2

验证数据展示

  • WB analysis of BxPC-3 using 14328-1-AP

    WB analysis of BxPC-3 using 14328-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14328-1-AP (NOMO2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 14328-1-AP

    IHC staining of human pancreas cancer using 14328-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14328-1-AP (NOMO2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human pancreas cancer using 14328-1-AP

    IHC staining of human pancreas cancer using 14328-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14328-1-AP (NOMO2 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of BxPC-3 using 14328-1-AP

    IF Staining of BxPC-3 using 14328-1-AP

    Immunofluorescent analysis of BxPC-3 cells using 14328-1-AP (NOMO2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of HEK-293 using 14328-1-AP

    IP experiment of HEK-293 using 14328-1-AP

    IP result of anti-NOMO2 (IP:14328-1-AP, 4ug; Detection:14328-1-AP 1:1000) with HEK-293 cells lysate 2400ug.

经过测试的应用

Positive WB detected inBxPC-3 cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inBxPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14328-1-AP targets NOMO2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen NOMO2 fusion protein Ag5595 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NODAL modulator 2
Synonyms pM5 protein 2, Nodal modulator 2, BOS complex subunit NOMO2
Calculated Molecular Weight 139 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC041131
Gene Symbol NOMO2
Gene ID (NCBI) 283820
RRIDAB_2152154
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5JPE7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NOMO2 antibody 14328-1-APDownload protocol
IHC protocol for NOMO2 antibody 14328-1-APDownload protocol
IF protocol for NOMO2 antibody 14328-1-APDownload protocol
IP protocol for NOMO2 antibody 14328-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Biol Ther

Radiotherapy diagnostic biomarkers in radioresistant human H460 lung cancer stem-like cells.

Authors - Hong Shik Yun
View Article