验证数据展示
经过测试的应用
| Positive WB detected in | C2C12 cell, mouse skeletal muscle tissue, ROS1728 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
66361-1-Ig targets NONO in WB, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | mouse |
| Immunogen | NONO fusion protein Ag17609 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | non-POU domain containing, octamer-binding |
| Synonyms | 55 kDa nuclear protein, NONO, NonO protein, NRB54, P54, p54(nrb), P54NRB |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 54 kDa |
| GenBank Accession Number | BC010049 |
| Gene Symbol | NONO |
| Gene ID (NCBI) | 4841 |
| RRID | AB_2881741 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q15233 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Non-pou domain-containing octamer-binding protein(NONO), other named p54nrb, belongs to DBHS family, whose protein are predominately nuclear and involves in transcriptional and posttranscriptional gene regulatory functions as well as DNA repair. This family has roles in a wide range of biological processes, such as the regulation of circadian rhythm, carcinogenesis and progression of cancer. NONO forms heterodimer with SFPQ, and has been implicated in nuclear retention of A- to I-edited RNA as paraspeckle components, pre-mRAN 3'-end formation, cAMP cycling and transcriptional activation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NONO antibody 66361-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
