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验证数据展示

  • WB analysis of MDA-MB-453s using 26854-1-AP

    WB analysis of MDA-MB-453s using 26854-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 26854-1-AP (NOP14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 26854-1-AP

    IF Staining of HepG2 using 26854-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 26854-1-AP (NOP14 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

NOP14 Polyclonal antibody

NOP14 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26854-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA and More (2)

别名

C4orf9, NOL14, NOP14, Nucleolar complex protein 14, Nucleolar protein 14, RES4 25, RES425

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMDA-MB-453s cells, BxPC-3 cells, MCF-7 cells, mouse lung tissue, rat kidney tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26854-1-AP targets NOP14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IF, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 NOP14 fusion protein Ag25476 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NOP14 nucleolar protein homolog (yeast)
别名 C4orf9, NOL14, NOP14, Nucleolar complex protein 14, Nucleolar protein 14, RES4 25, RES425
计算分子量 98 kDa
观测分子量 98 kDa
GenBank蛋白编号BC026035
基因名称 NOP14
Gene ID (NCBI) 8602
RRIDAB_2880657
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP78316
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NOP14 antibody 26854-1-APDownload protocol
IF protocol for NOP14 antibody 26854-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

Cell Prolif

CCND1, NOP14 and DNMT3B are involved in miR-502-5p-mediated inhibition of cell migration and proliferation in bladder cancer.

Authors - Yufan Ying
  • KD Validated
View Article
humanWB,IHC,IF

Aging (Albany NY)

Exploring Cancer Dependency Map genes and immune subtypes in colon cancer, in which TIGD1 contributes to colon cancer progression

Authors - Guoyang Zhang
View Article
humanWB

Int J Biol Macromol

CircNOP14 increases the radiosensitivity of hepatocellular carcinoma via inhibition of Ku70-dependent DNA damage repair

Authors - Letao Lin
View Article
humanWB,IHC

Front Biosci (Landmark Ed)

NOP14 as a Potential Predictor of Adult-Type Diffuse Glioma Prognosis and Immunotherapy, is Related to Cell Migration, Proliferation, and CD8+T Cell Infiltration

Authors - Qiu-Si Tian
View Article