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验证数据展示

  • WB analysis of NIH/3T3 using 18181-1-AP

    WB analysis of NIH/3T3 using 18181-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18181-1-AP (NOP56 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 18181-1-AP

    WB analysis using 18181-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18181-1-AP (NOP56 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 18181-1-AP

    IHC staining of human stomach using 18181-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18181-1-AP (NOP56 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NOP56 Polyclonal antibody

NOP56 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18181-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

NOP 56, NOL5A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, A549 cells, HeLa cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

18181-1-AP targets NOP56 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 NOP56 fusion protein Ag12882 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NOP56 ribonucleoprotein homolog (yeast)
别名 NOP 56, NOL5A
计算分子量 594 aa, 66 kDa
观测分子量 66 kDa
GenBank蛋白编号BC104791
基因名称 NOP56
Gene ID (NCBI) 10528
RRIDAB_2152323
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00567
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Box C/D RNA-protein complexes (RNPs) guide the 2'-O-methylation of nucleotides in eukaryotic ribosomal RNAs. The box C/D and C'/D' RNP subcomplexes are each assembled with three sRNP core proteins. The Nop56 core protein mediates crucial protein-protein interactions required for both sRNP assembly and the methyltransferase reaction by bridging the L7Ae and fibrillarin core proteins [PMID:22496443]. NOP56 is a component of the box C/D small nucleolar ribonucleoprotein complexes that direct 2-prime-O-methylation of pre-ribosomal RNA (rRNA) during its maturation. It is predicted to function in an early to middle step in pre-rRNA processing [PMID:12777385].

实验方案

Product Specific Protocols
WB protocol for NOP56 antibody 18181-1-APDownload protocol
IHC protocol for NOP56 antibody 18181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Biol Sci

Discovery of a novel HDACi structure that inhibits the proliferation of ovarian cancer cells in vivo and in vitro.

Authors - Miao Bai
View Article
mouseWB

iScience

BMAL1 Associates with NOP58 in the Nucleolus and Contributes to Pre-rRNA Processing.

Authors - Marlene Cervantes
View Article