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  • Featured Product
  • KD/KO Validated

NOSIP Polyclonal antibody

NOSIP Polyclonal Antibody for WB, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IF/ICC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 27979-1-AP

Print datasheet

Synonyms

CGI 25, eNOS interacting protein, NOSIP

验证数据展示

  • WB analysis using 27979-1-AP

    WB analysis using 27979-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27979-1-AP (NOSIP antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis using 27979-1-AP

    WB analysis using 27979-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27979-1-AP (NOSIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 27979-1-AP

    IHC staining of human colon using 27979-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27979-1-AP (NOSIP antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 27979-1-AP

    IHC staining of human colon using 27979-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27979-1-AP (NOSIP antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 27979-1-AP

    IF Staining of HeLa using 27979-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 27979-1-AP (NOSIP antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293T cells, A431 cells, A549 cells, Ramos cells, HepG2 cells, L02 cells
Positive IHC detected inhuman colon tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27979-1-AP targets NOSIP in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity Human
Cited Reactivityhuman
Immunogen NOSIP fusion protein Ag27569 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nitric oxide synthase interacting protein
Synonyms CGI 25, eNOS interacting protein, NOSIP
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC010077
Gene Symbol NOSIP
Gene ID (NCBI) 51070
RRIDAB_2881027
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y314
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NOSIP antibody 27979-1-APDownload protocol
IHC protocol for NOSIP antibody 27979-1-APDownload protocol
IF protocol for NOSIP antibody 27979-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

iScience

Nosip is a potential therapeutic target in hepatocellular carcinoma cells

Authors - Junjie Gao
  • KD Validated
View Article