验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse heart tissue, human placenta tissue, mouse liver tissue, mouse lung tissue |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human heart tissue, mouse testis tissue, human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20116-1-AP targets NOSTRIN in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | NOSTRIN fusion protein Ag13989 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nitric oxide synthase trafficker |
| 别名 | eNOS-trafficking inducer, eNOS trafficking inducer, DaIP2, BM247 homolog |
| 计算分子量 | 506 aa, 58 kDa |
| 观测分子量 | 64 kDa |
| GenBank蛋白编号 | BC014189 |
| 基因名称 | NOSTRIN |
| Gene ID (NCBI) | 115677 |
| RRID | AB_10666167 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IVI9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NOSTRIN is classically known as an endothelial cell protein that sequesters eNOS and inhibits NO production. NOSTRIN contains a single polypeptide chain of 506-aa residues of 58 kDa with an N-terminal cdc15 domain and a C-terminal SH3 domain. NOSTRIN mRNA is abundant in highly vascularized tissues such as placenta, kidney, lung, and heart, and NOSTRIN protein is expressed in vascular endothelial cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NOSTRIN antibody 20116-1-AP | Download protocol |
| IHC protocol for NOSTRIN antibody 20116-1-AP | Download protocol |
| IF protocol for NOSTRIN antibody 20116-1-AP | Download protocol |
| IP protocol for NOSTRIN antibody 20116-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Redox Biol Cardioprotection of CAPE-oNO2 against myocardial ischemia/reperfusion induced ROS generation via regulating the SIRT1/eNOS/NF-κB pathway in vivo and in vitro. | ||
J Inflamm Res TNF-α and IL-1β Promote Renal Podocyte Injury in T2DM Rats by Decreasing Glomerular VEGF/eNOS Expression Levels and Altering Hemodynamic Parameters | ||
Naunyn Schmiedebergs Arch Pharmacol Thymoquinone mitigate ischemia-reperfusion-induced liver injury in rats: a pivotal role of nitric oxide signaling pathway. | ||
Mol Med Rep Appropriate timing for hypothermic machine perfusion to preserve livers donated after circulatory death. | ||
Mol Med Rep UBIAD1 expression is associated with cardiac hypertrophy in spontaneously hypertensive rats. | ||
Mol Med Rep Depletion of ubiA prenyltransferase domain containing 1 expression promotes angiotensin II‑induced hypertrophic response in AC16 human myocardial cells via modulating the expression levels of coenzyme Q10 and endothelial nitric oxide synthase. |
