NOTCH2 Recombinant antibody
NOTCH2 Uni-rAbTM Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
241162A2
验证数据展示
经过测试的应用
| Positive WB detected in | U-87 MG cells, HEK-293 cells, A431 cells, HeLa cells, MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy NOTCH2 Ready-To-Use IHC Kit. NOTCH2 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 84074-4-RR is NOTCH2 Fusion Protein expressed in E. coli.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | NOTCH2 fusion protein Ag29413 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | Notch homolog 2 (Drosophila) |
| Synonyms | |
| Calculated Molecular Weight | 265 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | NM_024408 |
| Gene Symbol | NOTCH2 |
| Gene ID (NCBI) | 4853 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q04721 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NOTCH2 (Neurogenic locus notch homolog protein 2) belongs to the NOTCH family.. In kidney, previous reports demonstrated that Notch1 and Notch2 were activated in mammalian nephrogenesis (PMID: 24526233). Notch2 is expressed in many cell types of most lineages in the hematolymphoid compartment and has specific roles in differentiation and function of various immune cells. Notch2 is required for development of splenic marginal zone B cells and regulates differentiation of dendritic cells (DCs) in the spleen. Notch2 appears to play some specific roles in the intestinal immunity, given that the fate of mast cells and a subset of DCs is regulated by Notch2 in the intestine. Notch2 also has important roles in helper T cell divergence from naïve CD4 T cells and activation of cytotoxic T cells (PMID: 22695918).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NOTCH2 antibody 84074-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |