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验证数据展示

  • WB analysis of hTERT-RPE1 using 68212-1-Ig

    WB analysis of hTERT-RPE1 using 68212-1-Ig

    hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68212-1-Ig (NOTCH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 68212-1-Ig

    IF Staining of HeLa using 68212-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NOTCH2/NOTCH2NL antibody (68212-1-Ig, Clone: 2D5E1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

NOTCH2/NOTCH2NL Monoclonal antibody

NOTCH2/NOTCH2NL Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68212-1-Ig

产品说明书

CloneNo. 2D5E1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

NOTCH2NL, NOTCH2NLC, NOTCH2, Notch homolog 2 N-terminal-like protein C, N2N

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhTERT-RPE1 cells, U-87 MG cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68212-1-Ig targets NOTCH2/NOTCH2NL in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 NOTCH2/NOTCH2NL fusion protein Ag1679 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Notch homolog 2 (Drosophila) N-terminal like
别名 NOTCH2NL, NOTCH2NLC, NOTCH2, Notch homolog 2 N-terminal-like protein C, N2N
计算分子量 265 kDa
观测分子量250-265 kDa
GenBank蛋白编号BC019835
基因名称 NOTCH2NL
Gene ID (NCBI) 388677
RRIDAB_2935300
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP0DPK4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Notch 2 is a member of the notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. Notch2, like other Norch genes, functions in development of various tissues. This antibody can recognize NOTCH2 and NOTCH2NL. The antibody detects the full-length NOTCH2 protein about 250-265 kDa.

实验方案

Product Specific Protocols
WB protocol for NOTCH2/NOTCH2NL antibody 68212-1-IgDownload protocol
IF protocol for NOTCH2/NOTCH2NL antibody 68212-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols