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验证数据展示

  • WB analysis of hTERT-RPE1 using 68212-1-Ig

    WB analysis of hTERT-RPE1 using 68212-1-Ig

    hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68212-1-Ig (NOTCH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 68212-1-Ig

    IF Staining of HeLa using 68212-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NOTCH2/NOTCH2NL antibody (68212-1-Ig, Clone: 2D5E1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

NOTCH2/NOTCH2NL Monoclonal antibody

NOTCH2/NOTCH2NL Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68212-1-Ig

Print datasheet

CloneNo. 2D5E1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

NOTCH2NL, NOTCH2NLC, NOTCH2, Notch homolog 2 N-terminal-like protein C, N2N

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhTERT-RPE1 cells, U-87 MG cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68212-1-Ig targets NOTCH2/NOTCH2NL in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen NOTCH2/NOTCH2NL fusion protein Ag1679 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Notch homolog 2 (Drosophila) N-terminal like
Synonyms NOTCH2NL, NOTCH2NLC, NOTCH2, Notch homolog 2 N-terminal-like protein C, N2N
Calculated Molecular Weight 265 kDa
Observed Molecular Weight250-265 kDa
GenBank Accession NumberBC019835
Gene Symbol NOTCH2NL
Gene ID (NCBI) 388677
RRIDAB_2935300
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP0DPK4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Notch 2 is a member of the notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. Notch2, like other Norch genes, functions in development of various tissues. This antibody can recognize NOTCH2 and NOTCH2NL. The antibody detects the full-length NOTCH2 protein about 250-265 kDa.

实验方案

Product Specific Protocols
WB protocol for NOTCH2/NOTCH2NL antibody 68212-1-IgDownload protocol
IF protocol for NOTCH2/NOTCH2NL antibody 68212-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols