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验证数据展示

  • WB analysis using 30540-1-AP

    WB analysis using 30540-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30540-1-AP (NOTCH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 30540-1-AP

    IP experiment of HeLa using 30540-1-AP

    IP result of anti-NOTCH3 (IP:30540-1-AP, 4ug; Detection:30540-1-AP 1:1000) with HeLa cells lysate 1320 ug.

NOTCH3 Polyclonal antibody

NOTCH3 Polyclonal Antibody for WB, IP, ELISA
Cat No. 30540-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IP, ELISA

别名

CADASIL, CASIL, Notch 3, Notch homolog 3 (Drosophila), NOTCH3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCaco-2 cells, HeLa cells, U2OS cells, mouse lung tissue
Positive IP detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30540-1-AP targets NOTCH3 in WB, IP, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 Human, mouse
免疫原 NOTCH3 fusion protein Ag30242 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Notch homolog 3 (Drosophila)
别名 CADASIL, CASIL, Notch 3, Notch homolog 3 (Drosophila), NOTCH3
计算分子量 244 kDa
观测分子量 270 kDa, 90 kDa
GenBank蛋白编号NM_000435
基因名称 NOTCH3
Gene ID (NCBI) 4854
RRIDAB_3086356
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UM47
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NOTCH3 (Neurogenic locus notch homolog protein 3) is one of four mammalian Notch proteins, which act as signalling receptors to control cell fate in many developmental and adult tissue contexts (PMID: 32210034). Notch is a transmembrane, developmental signalling receptor, which plays many crucial roles in developmental patterning, cell fate decisions, regulation of cell survival and proliferation (PMID:30246579). Genetic studies have shown that Notch3 gene knockouts are viable and have limited developmental defects, focussed mostly on defects in the arterial smooth muscle cell lineage. Notch3, in collaboration with other Notch proteins, is involved in stem cell regulation in different tissues in stem cell regulation in different tissues, and it also controls the plasticity of the vascular smooth muscle phenotype involved in arterial vessel remodelling. Overexpression, gene amplification and mis-activation of Notch3 are associated with different cancers, in particular triple negative breast cancer and ovarian cancer (PMID: 32210034).

实验方案

Product Specific Protocols
WB protocol for NOTCH3 antibody 30540-1-APDownload protocol
IP protocol for NOTCH3 antibody 30540-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols