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NOTUM Monoclonal antibody

NOTUM Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F6D5

Cat no : 66956-1-Ig

Print datasheet

Synonyms

NOTUM, Protein notum homolog

验证数据展示

  • WB analysis using 66956-1-Ig

    WB analysis using 66956-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66956-1-Ig (NOTUM antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 66956-1-Ig

    IHC staining of human small intestine using 66956-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66956-1-Ig (NOTUM antibody) at dilution of 1:5000 (under 10x lens)..

  • IHC staining of human small intestine using 66956-1-Ig

    IHC staining of human small intestine using 66956-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66956-1-Ig (NOTUM antibody) at dilution of 1:5000 (under 40x lens)..

经过测试的应用

Positive WB detected inMCF-7 cells, HepG2 cells, T-47D cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66956-1-Ig targets NOTUM in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen NOTUM fusion protein Ag6338 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name notum pectinacetylesterase homolog (Drosophila)
Synonyms NOTUM, Protein notum homolog
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC060882
Gene Symbol NOTUM
Gene ID (NCBI) 147111
RRIDAB_2882279
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ6P988
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NOTUM antibody 66956-1-IgDownload protocol
IHC protocol for NOTUM antibody 66956-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols