验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, MCF-7 cells, HEK-293 cells, PC-12 cells, mouse kidney tissue, mouse liver tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
30131-1-AP targets NOX3 in WB, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human, mouse |
| Cited Reactivity | pig |
| Immunogen | NOX3 fusion protein Ag32601 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | NADPH oxidase 3 |
| Synonyms | GP91 3, gp91phox homolog 3, Mitogenic oxidase 2, MOX 2, MOX2, NADPH oxidase 3, NOX3 |
| Calculated Molecular Weight | 64kd |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | NM_015718 |
| Gene Symbol | NOX3 |
| Gene ID (NCBI) | 50508 |
| RRID | AB_2935517 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HBY0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NOX3 is a member of NADPH oxidase which constitutively produces superoxide upon formation of a complex with CYBA/p22phox. NOX3 plays a role in the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. The antibody is specific to NOX3.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NOX3 antibody 30131-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |