验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, MCF-7 cells, HEK-293 cells, PC-12 cells, mouse kidney tissue, mouse liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
30131-1-AP targets NOX3 in WB, ELISA applications and shows reactivity with Human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, mouse |
| 文献引用反应性 | pig |
| 免疫原 | NOX3 fusion protein Ag32601 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NADPH oxidase 3 |
| 别名 | GP91 3, gp91phox homolog 3, Mitogenic oxidase 2, MOX 2, MOX2, NADPH oxidase 3, NOX3 |
| 计算分子量 | 64kd |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | NM_015718 |
| 基因名称 | NOX3 |
| Gene ID (NCBI) | 50508 |
| RRID | AB_2935517 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HBY0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NOX3 is a member of NADPH oxidase which constitutively produces superoxide upon formation of a complex with CYBA/p22phox. NOX3 plays a role in the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. The antibody is specific to NOX3.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NOX3 antibody 30131-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |