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验证数据展示

  • IHC staining of human brain using 25517-1-AP

    IHC staining of human brain using 25517-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25517-1-AP (NPB Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 25517-1-AP

    IHC staining of human brain using 25517-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25517-1-AP (NPB Antibody) at dilution of 1:50 (under 40x lens).

NPB Polyclonal antibody

NPB Polyclonal Antibody for IHC, ELISA
Cat No. 25517-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

hL7 Neuropeptide B 29, hL7C, hPPL7, neuropeptide B, NPB, PPL7, PPNPB, Preproprotein L7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inhuman brain tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25517-1-AP targets NPB in IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human
Immunogen NPB fusion protein Ag21877 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neuropeptide B
Synonyms hL7 Neuropeptide B 29, hL7C, hPPL7, neuropeptide B, NPB, PPL7, PPNPB, Preproprotein L7
Calculated Molecular Weight 125 aa, 13 kDa
Observed Molecular Weight ~10 kDa
GenBank Accession NumberBC126128
Gene Symbol NPB
Gene ID (NCBI) 256933
RRIDAB_2880114
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NG41
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuropeptide B, also named as NPB, PPL7 and PPNBP, can be cleaved into two chains, Neuropeptide B-23 (NPB23, hL7) and Neuropeptide B-29 (NPB29, hL7C). It is involved in the regulation of feeding, neuroendocrine system, memory, learning and in the afferent pain pathway. It is widely expressed in central nervous system.

实验方案

Product Specific Protocols
IHC protocol for NPB antibody 25517-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols