验证数据展示
经过测试的应用
Positive IHC detected in | human brain tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
25517-1-AP targets NPB in IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | IHC, ELISA Application Description |
经测试反应性 | human |
免疫原 | NPB fusion protein Ag21877 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | neuropeptide B |
别名 | hL7 Neuropeptide B 29, hL7C, hPPL7, neuropeptide B, NPB, PPL7, PPNPB, Preproprotein L7 |
计算分子量 | 125 aa, 13 kDa |
观测分子量 | ~10 kDa |
GenBank蛋白编号 | BC126128 |
基因名称 | NPB |
Gene ID (NCBI) | 256933 |
RRID | AB_2880114 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q8NG41 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Neuropeptide B, also named as NPB, PPL7 and PPNBP, can be cleaved into two chains, Neuropeptide B-23 (NPB23, hL7) and Neuropeptide B-29 (NPB29, hL7C). It is involved in the regulation of feeding, neuroendocrine system, memory, learning and in the afferent pain pathway. It is widely expressed in central nervous system.
实验方案
Product Specific Protocols | |
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IHC protocol for NPB antibody 25517-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |