NPHP5/IQCB1 Polyclonal antibody
NPHP5/IQCB1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
15747-1-AP targets NPHP5/IQCB1 in WB, IF, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | IF |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | NPHP5/IQCB1 fusion protein Ag8203 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | IQ motif containing B1 |
| Synonyms | IQ motif containing B1, IQCB1, KIAA0036, Nephrocystin 5, NPHP5, NPHP5,IQCB1, PIQ, SLSN5 |
| Calculated Molecular Weight | 598 aa, 69 kDa |
| Observed Molecular Weight | 69 kDa |
| GenBank Accession Number | BC005806 |
| Gene Symbol | IQCB1 |
| Gene ID (NCBI) | 9657 |
| RRID | AB_2878179 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15051 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IQCB1, also known as NPHP5, is a nephrocystin protein that interacts with calmodulin and the retinitis pigmentosa GTPase regulator protein. It has a central coiled-coil region and two calmodulin-binding IQ domains. Localized to the primary cilia of renal epithelial cells and connecting cilia of photoreceptor cells, IQCB1 is thought to play a role in ciliary function. Mutations in this gene result in Senior-Loken syndrome type 5, a juvenile disorder characterized by defects in the waste filtering system of the kidney, as well as retinal degradation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NPHP5/IQCB1 antibody 15747-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Hum Mol Genet Pathogenic NPHP5 mutations impair protein interaction with Cep290, a prerequisite for ciliogenesis. | ||
Mol Biol Cell Rpgrip1l controls ciliary gating by ensuring the proper amount of Cep290 at the vertebrate transition zone. |