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  • Featured Product
  • KD/KO Validated

NPR1 Polyclonal antibody

NPR1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55116-1-AP

Print datasheet

Synonyms

ANP A, ANPa, ANPR A, ANPRA, GC A, Guanylate cyclase A, GUC2A, GUCY2A, NPR1

验证数据展示

  • WB analysis of SGC-7901 using 55116-1-AP

    WB analysis of SGC-7901 using 55116-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 55116-1-AP (NPR1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 55116-1-AP

    IHC staining of human stomach cancer using 55116-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55116-1-AP (NPR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 55116-1-AP

    IHC staining of human stomach cancer using 55116-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55116-1-AP (NPR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inSGC-7901 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55116-1-AP targets NPR1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A)
Synonyms ANP A, ANPa, ANPR A, ANPRA, GC A, Guanylate cyclase A, GUC2A, GUCY2A, NPR1
Calculated Molecular Weight 119 kDa
Observed Molecular Weight 119 kDa
GenBank Accession NumberNM_000906
Gene Symbol NPR1
Gene ID (NCBI) 4881
RRIDAB_2881271
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16066
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NPR1 antibody 55116-1-APDownload protocol
IHC protocol for NPR1 antibody 55116-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

17β-Estradiol nongenomically induces vascular endothelial H2S release by promoting phosphorylation of cystathionine γ-lyase.

Authors - Xingyan Xu
View Article
humanWB

Int J Clin Exp Med

Impact of NPR-A expression in gastric cancer cells.

Authors - Jia Zhang
  • KD Validated
View Article