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NPSR1 Polyclonal antibody

NPSR1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 25085-1-AP

Print datasheet

Synonyms

neuropeptide S receptor 1, Neuropeptide S receptor, GPRA, GPR154, G protein coupled receptor 154

验证数据展示

  • IHC staining of human colon cancer using 25085-1-AP

    IHC staining of human colon cancer using 25085-1-AP

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 25085-1-AP (NPSR1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 25085-1-AP

    IHC staining of human cervical cancer using 25085-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25085-1-AP (NPSR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human endometrial cancer using 25085-1-AP

    IHC staining of human endometrial cancer using 25085-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25085-1-AP (NPSR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25085-1-AP targets NPSR1 in IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human
Immunogen NPSR1 fusion protein Ag18785 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neuropeptide S receptor 1
Synonyms neuropeptide S receptor 1, Neuropeptide S receptor, GPRA, GPR154, G protein coupled receptor 154
Calculated Molecular Weight 371 aa, 43 kDa
GenBank Accession NumberBC132693
Gene Symbol NPSR1
Gene ID (NCBI) 387129
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6W5P4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuropeptide S receptor 1 (NPSR1), previously known as GPRA and GPR154, is a G protein-coupled receptor (GPCR) that plays a significant role in various physiological processes. NPSR1 is primarily expressed in the bronchus, brain, and immune cells. It is also found in specific peripheral cell types such as monocytes/macrophages and neuroendocrine cells of the gut (PMID: 30711025).

实验方案

Product Specific Protocols
IHC protocol for NPSR1 antibody 25085-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols