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NPTN Polyclonal antibody

NPTN Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28022-1-AP

Print datasheet

Synonyms

DKFZp686L2477, GP55, GP65, neuroplastin, NPTN, SDFR1, SDR 1, SDR1

验证数据展示

  • WB analysis of C6 using 28022-1-AP

    WB analysis of C6 using 28022-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 28022-1-AP (NPTN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inC6 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28022-1-AP targets NPTN in WB, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, rat
Immunogen NPTN fusion protein Ag27752 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neuroplastin
Synonyms DKFZp686L2477, GP55, GP65, neuroplastin, NPTN, SDFR1, SDR 1, SDR1
Calculated Molecular Weight 282 aa, 31 kDa
Observed Molecular Weight65 kDa
GenBank Accession NumberBC030773
Gene Symbol NPTN
Gene ID (NCBI) 27020
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y639
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuroplastin (Nptn) is a member of the Ig superfamily and is expressed in two isoforms, Np55 and Np65. Np65 contains three Ig domains (Ig1, Ig2, Ig3). Np55 contains only two (Ig2, Ig3). Np65 regulates synaptic transmission but the function of Np55 is unknown. Expression of the 65 kD neuroplastin (Np65) isoform derived by alternative splicing from the Nptn gene is restricted to brain neurons, whereas the 55 kD isoform (Np55) is present in most cell types but not in glia. (PMID: 33844052, PMID: 33299977, PMID: 27503893,PMID: 27581460)

实验方案

Product Specific Protocols
WB protocol for NPTN antibody 28022-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols