验证数据展示
经过测试的应用
| Positive WB detected in | C6 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28022-1-AP targets NPTN in WB, ELISA applications and shows reactivity with Human, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, rat |
| 免疫原 | NPTN fusion protein Ag27752 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | neuroplastin |
| 别名 | DKFZp686L2477, GP55, GP65, neuroplastin, NPTN, SDFR1, SDR 1, SDR1 |
| 计算分子量 | 282 aa, 31 kDa |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | BC030773 |
| 基因名称 | NPTN |
| Gene ID (NCBI) | 27020 |
| RRID | AB_3086022 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y639 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Neuroplastin (Nptn) is a member of the Ig superfamily and is expressed in two isoforms, Np55 and Np65. Np65 contains three Ig domains (Ig1, Ig2, Ig3). Np55 contains only two (Ig2, Ig3). Np65 regulates synaptic transmission but the function of Np55 is unknown. Expression of the 65 kD neuroplastin (Np65) isoform derived by alternative splicing from the Nptn gene is restricted to brain neurons, whereas the 55 kD isoform (Np55) is present in most cell types but not in glia. (PMID: 33844052, PMID: 33299977, PMID: 27503893,PMID: 27581460)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NPTN antibody 28022-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |