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NQO1 Monoclonal antibody, PBS Only

NQO1 Monoclonal Antibody for Indirect ELISA, IHC, WB

Host / Isotype

Mouse / IgG2a

Reactivity

rat, human

Applications

Indirect ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

1E5G7

Cat no : 67240-1-PBS

Print datasheet

Synonyms

DT diaphorase, DIA4, DIA 4, DHQU, Azoreductase

验证数据展示

  • WB analysis using 67240-1-Ig (same clone as 67240-1-PBS)

    WB analysis using 67240-1-Ig (same clone as 67240-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67240-1-Ig (NQO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 67240-1-Ig (same clone as 67240-1-PBS)

    IHC staining of human colon using 67240-1-Ig (same clone as 67240-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67240-1-Ig (NQO1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 67240-1-Ig (same clone as 67240-1-PBS)

    IHC staining of human colon using 67240-1-Ig (same clone as 67240-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67240-1-Ig (NQO1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 67240-1-Ig (same clone as 67240-1-PBS)

    FC experiment of MCF-7 using 67240-1-Ig (same clone as 67240-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human NQO1 (67240-1-Ig, Clone:1E5G7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

产品信息

67240-1-PBS targets NQO1 in Indirect ELISA, IHC, WB applications and shows reactivity with rat, human samples.

Tested Applications Indirect ELISA, IHC, WB Application Description
Tested Reactivity rat, human
Immunogen NQO1 fusion protein Ag28933 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name NAD(P)H dehydrogenase, quinone 1
Synonyms DT diaphorase, DIA4, DIA 4, DHQU, Azoreductase
Calculated Molecular Weight 274 aa, 31 kDa
Observed Molecular Weight 29-31 kDa
GenBank Accession NumberBC007659
Gene Symbol NQO1
Gene ID (NCBI) 1728
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15559
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 )