验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, rabbit kidney tissue, HeLa, K-562, TF-1 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68487-1-Ig targets NQO2 in WB, ELISA applications and shows reactivity with Human, mouse, rabbit samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | Human, mouse, rabbit |
免疫原 | NQO2 fusion protein Ag8156 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | NAD(P)H dehydrogenase, quinone 2 |
别名 | DHQV, DIA6, NQO2, NRH dehydrogenase [quinone] 2, NRH:quinone oxidoreductase 2, QR2, Quinone reductase 2 |
计算分子量 | 231 aa, 26 kDa |
GenBank蛋白编号 | BC006096 |
基因名称 | NQO2 |
Gene ID (NCBI) | 4835 |
RRID | AB_3085198 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P16083 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NQO2, also named as QR2 and NMOR2, belongs to the NAD(P)H dehydrogenase (quinone) family. It serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. The cytosolic quinone oxidoreductases NQO1 and NQO2 protect cells against oxidative stress by detoxifying quinones and preventing redox cycling. NQO1 and NQO2 are important endogenous factors in regulation of immune response and autoimmunity.
实验方案
Product Specific Protocols | |
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WB protocol for NQO2 antibody 68487-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |