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验证数据展示

  • WB analysis of rabbit kidney using 68487-1-Ig

    WB analysis of rabbit kidney using 68487-1-Ig

    rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68487-1-Ig (NQO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68487-1-Ig

    WB analysis using 68487-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68487-1-Ig (NQO2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

NQO2 Monoclonal antibody

NQO2 Monoclonal Antibody for WB, ELISA
Cat No. 68487-1-Ig

Print datasheet

CloneNo. 1D9B5

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rabbit

Applications

WB, ELISA

Synonyms

DHQV, DIA6, NQO2, NRH dehydrogenase [quinone] 2, NRH:quinone oxidoreductase 2, QR2, Quinone reductase 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, rabbit kidney tissue, HeLa, K-562, TF-1

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68487-1-Ig targets NQO2 in WB, ELISA applications and shows reactivity with Human, mouse, rabbit samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rabbit
Immunogen NQO2 fusion protein Ag8156 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name NAD(P)H dehydrogenase, quinone 2
Synonyms DHQV, DIA6, NQO2, NRH dehydrogenase [quinone] 2, NRH:quinone oxidoreductase 2, QR2, Quinone reductase 2
Calculated Molecular Weight 231 aa, 26 kDa
GenBank Accession NumberBC006096
Gene Symbol NQO2
Gene ID (NCBI) 4835
RRIDAB_3085198
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP16083
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NQO2, also named as QR2 and NMOR2, belongs to the NAD(P)H dehydrogenase (quinone) family. It serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. The cytosolic quinone oxidoreductases NQO1 and NQO2 protect cells against oxidative stress by detoxifying quinones and preventing redox cycling. NQO1 and NQO2 are important endogenous factors in regulation of immune response and autoimmunity.

实验方案

Product Specific Protocols
WB protocol for NQO2 antibody 68487-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols