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- KD/KO Validated
NR1D1 Polyclonal antibody
NR1D1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IF/ICC, IP, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HepG2 cells, mouse skeletal muscle tissue, Jurkat cells, NIH/3T3 cells, Y79 cells, HeLa cells |
| Positive IP detected in | A549 cells |
| Positive IF/ICC detected in | NIH/3T3 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14506-1-AP targets NR1D1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, ChIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Immunogen | NR1D1 fusion protein Ag5964 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | nuclear receptor subfamily 1, group D, member 1 |
| Synonyms | Reverb alpha, Rev erbA alpha, HREV, EAR-1, EAR1 |
| Calculated Molecular Weight | 67 kDa |
| Observed Molecular Weight | 55-68 kDa |
| GenBank Accession Number | BC056148 |
| Gene Symbol | NR1D1 |
| Gene ID (NCBI) | 9572 |
| RRID | AB_11182604 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20393 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NR1D1, also named EAR1, THRAL, ear-1, and hRev, contains one nuclear receptor DNA-binding domain and belongs to the nuclear hormone receptor family. NR1 subfamily. This protein functions as a constitutive transcriptional repressor. It is a possible receptor for triiodothyronine. BMAL1-CLOCK activates Rev-erba itself and thereby represents the link between the positive and negative loops of the circadian clock. This is a rabbit polyclonal antibody raised against the N terminus of human NR1D1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NR1D1 antibody 14506-1-AP | Download protocol |
| IF protocol for NR1D1 antibody 14506-1-AP | Download protocol |
| IP protocol for NR1D1 antibody 14506-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock. | ||
Theranostics PPAR-γ integrates obesity and adipocyte clock through epigenetic regulation of Bmal1. | ||
Cancer Res NR1D1 recruitment to sites of DNA damage inhibits repair and is associated with chemosensitivity of breast cancer. | ||
Cell Death Discov NR1D1 deficiency in the tumor microenvironment promotes lung tumor development by activating the NLRP3 inflammasome | ||
J Neuroinflammation The circadian clock protein Rev-erbα provides neuroprotection and attenuates neuroinflammation against Parkinson's disease via the microglial NLRP3 inflammasome. | ||
Cell Biosci Rev-erbα exacerbates hepatic steatosis in alcoholic liver diseases through regulating autophagy. |
