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- KD/KO Validated
NR1H4 Polyclonal antibody
NR1H4 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IHC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, MCF-7 cells, MDA-MB-231 cells |
Positive IP detected in | rat liver tissue |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
25055-1-AP targets NR1H4 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, CoIP |
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat, pig |
Immunogen | NR1H4 fusion protein Ag21878 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | nuclear receptor subfamily 1, group H, member 4 |
Synonyms | FXR, Farnesol receptor HRR-1, Farnesoid X-activated receptor, Farnesoid X activated receptor, Bile acid receptor |
Calculated Molecular Weight | 486 aa, 56 kDa |
Observed Molecular Weight | 48 kDa |
GenBank Accession Number | BC130573 |
Gene Symbol | NR1H4 |
Gene ID (NCBI) | 9971 |
RRID | AB_2879874 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q96RI1 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for NR1H4 antibody 25055-1-AP | Download protocol |
IHC protocol for NR1H4 antibody 25055-1-AP | Download protocol |
IP protocol for NR1H4 antibody 25055-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Host Microbe A gut microbiota-bile acid axis promotes intestinal homeostasis upon aspirin-mediated damage | ||
Cell Host Microbe Dietary fiber alleviates alcoholic liver injury via Bacteroides acidifaciens and subsequent ammonia detoxification
| ||
Sci Total Environ Bisphenol A induced hepatic steatosis by disturbing bile acid metabolism and FXR/TGR5 signaling pathways via remodeling the gut microbiota in CD-1 mice | ||
Phytomedicine Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis | ||
Phytomedicine Jiang-Tang-San-Huang pill alleviates type 2 diabetes mellitus through modulating the gut microbiota and bile acids metabolism | ||
Biomater Sci Mesenchymal stem cell-derived exosome mitigates colitis via the modulation of the gut metagenomics-metabolomics-farnesoid X receptor axis |