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NR2C1 Polyclonal antibody

NR2C1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat No : 13658-1-AP

Print datasheet

Synonyms

NR2C1, Orphan nuclear receptor TR2, Testicular receptor 2, TR2

验证数据展示

  • WB analysis of SH-SY5Y using 13658-1-AP

    WB analysis of SH-SY5Y using 13658-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13658-1-AP (NR2C1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 13658-1-AP

    IHC staining of human prostate cancer using 13658-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13658-1-AP (NR2C1 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

  • IHC staining of human prostate cancer using 13658-1-AP

    IHC staining of human prostate cancer using 13658-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13658-1-AP (NR2C1 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

经过测试的应用

Positive WB detected inSH-SY5Y cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13658-1-AP targets NR2C1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NR2C1 fusion protein Ag4554 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear receptor subfamily 2, group C, member 1
Synonyms NR2C1, Orphan nuclear receptor TR2, Testicular receptor 2, TR2
Calculated Molecular Weight 467 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC040141
Gene Symbol NR2C1
Gene ID (NCBI) 7181
RRIDAB_2236290
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13056
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear receptor subfamily 2 group C member 1 (NR2C1), also known as Orphan nuclear receptor TR2, is a member of the steroid/thyroid hormone receptor superfamily that controls a variety of processes, including growth, differentiation and development. TR2 is known to bind to regulatory elements of the erythropoietin gene, the muscle-specific aldolase A gene and the CNTF-15 gene. In addition to TR2, a related orphan receptor, designated TR4, has been identified. TR4 forms heterodimers with TR2, which are thought to be involved in neurogenesis and germ cell development. TR2 is known to be downregulated by both p53 and ionizing radiation, and it may play a role in linking p53 to members of the steroid recepr family.

实验方案

Product Specific Protocols
WB protocol for NR2C1 antibody 13658-1-APDownload protocol
IHC protocol for NR2C1 antibody 13658-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols