验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, COLO 320 cells, L02 cells, mouse liver tissue, rat liver tissue, PC-3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse colon tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
Planning an IHC experiment? We recommend our IHCeasy NR4A1 Ready-To-Use IHC Kit. NR4A1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12235-1-AP targets NR4A1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP, ELISA |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | NR4A1 fusion protein Ag2875 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nuclear receptor subfamily 4, group A, member 1 |
| 别名 | NGFIB, NAK1, NAK 1, N10, HMR |
| 计算分子量 | 598 aa, 64 kDa |
| 观测分子量 | 64-70 kDa |
| GenBank蛋白编号 | BC016147 |
| 基因名称 | NR4A1 |
| Gene ID (NCBI) | 3164 |
| RRID | AB_10644125 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P22736 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NR4A1 is an orphan nuclear receptor that act concomitantly with NURR1 in regulating the expression of delay-early genes during liver regeneration. Also NR4A1 has linked not only to cell apoptosis but also proliferation in response to growth factor. It plays a role in caspase-independent cell death by activation of the ERK pathway and increased activity of MEF2 transcription factors. NR4A1 exists some isoforms with MW 65 and 34 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NR4A1 antibody 12235-1-AP | Download protocol |
| IHC protocol for NR4A1 antibody 12235-1-AP | Download protocol |
| IF protocol for NR4A1 antibody 12235-1-AP | Download protocol |
| IP protocol for NR4A1 antibody 12235-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) A systematic dissection of human primary osteoblasts in vivo at single-cell resolution. | ||
Sci Rep Nur77 exacerbates PC12 cellular injury in vitro by aggravating mitochondrial impairment and endoplasmic reticulum stress.
| ||
Sci Rep NR4A1 Knockdown Suppresses Seizure Activity by Regulating Surface Expression of NR2B.
| ||
Mol Neurobiol Contra-directional Coupling of Nur77 and Nurr1 in Neurodegeneration: A Novel Mechanism for Memantine-Induced Anti-inflammation and Anti-mitochondrial Impairment. | ||
Bioorg Chem Structure-based design and synthesis of anti-fibrotic compounds derived from para-positioned 3,4,5-trisubstituted benzene | ||
Mol Neurobiol HPGD: An Intermediate Player in Microglial Polarization and Multiple Sclerosis Regulated by Nr4a1 |
