验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, PC-3 cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy NR4A1 Ready-To-Use IHC Kit. NR4A1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25851-1-AP targets NR4A1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | NR4A1 fusion protein Ag23088 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nuclear receptor subfamily 4, group A, member 1 |
| 别名 | Early response protein NAK1, GFRP1, HMR, N10, NAK 1, NAK1, NGFIB, NR4A1, NUR77, Orphan nuclear receptor HMR, Orphan nuclear receptor TR3, ST 59, Testicular receptor 3, TR3 |
| 计算分子量 | 598 aa, 64 kDa |
| 观测分子量 | 64-68 kDa |
| GenBank蛋白编号 | BC016147 |
| 基因名称 | NR4A1 |
| Gene ID (NCBI) | 3164 |
| RRID | AB_2880269 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P22736 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NR4A1 antibody 25851-1-AP | Download protocol |
| IHC protocol for NR4A1 antibody 25851-1-AP | Download protocol |
| IP protocol for NR4A1 antibody 25851-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Lett NR4A1 depletion inhibits colorectal cancer progression by promoting necroptosis via the RIG-I-like receptor pathway | ||
J Cell Mol Med NR4A1 retards adipocyte differentiation or maturation via enhancing GATA2 and p53 expression.
| ||
Phytomedicine Stigmasterol upregulates immediate early genes and promotes neuronal cytoarchitecture in primary hippocampal neurons as revealed by transcriptome analysis. | ||
Front Mol Biosci Single-Cell Profiles of Age-Related Osteoarthritis Uncover Underlying Heterogeneity Associated With Disease Progression.
| ||
Am J Physiol Endocrinol Metab Frequent and mild scrotal heat stress in mice epigenetically alters glucose metabolism in the male offspring. |
