验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, K-562 cells, mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12712-1-AP targets GCNF in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | GCNF fusion protein Ag3395 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nuclear receptor subfamily 6, group A, member 1 |
| 别名 | GCNF, GCNF1, Germ cell nuclear factor, hGCNF, hRTR, NR61, NR6A1, RTR |
| 计算分子量 | 475 aa, 54 kDa |
| 观测分子量 | 54 kDa |
| GenBank蛋白编号 | BC030600 |
| 基因名称 | GCNF |
| Gene ID (NCBI) | 2649 |
| RRID | AB_10640439 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15406 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NR6A1 (Nuclear Receptor Subfamily 6 Group A member), also known as GCNF (Germ Cell Nuclear Factor), is an orphan member of the nuclear receptor gene superfamily. It has been shown to be expressed in the nervous system during development and during specific stages in maturing germ cells of the ovary and testis in the adult, and has probable roles in gametogenesis, neurogenesis, and normal embryonic development during gastrulation. Inactivation of GCNF in mouse results in abnormal posterior development, impaired midbrain development, insufficient closure of the neural tube, and eventual embryonic death. GCNF has been shown to be a repressor of OCT-4 and plays a critical role in the control of gene expression during embryogenesis and spermatogenesis. This antibody specifically reacts with the 55kd NR6A1 protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GCNF antibody 12712-1-AP | Download protocol |
| IHC protocol for GCNF antibody 12712-1-AP | Download protocol |
| IP protocol for GCNF antibody 12712-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Neurobiol Mitofusin 2 Mediates the Protective Effect of NR6A1 Silencing Against Neuronal Injury in Experimental Stroke Models | ||
Neuropharmacology Hippocampal NR6A1 impairs CREB-BDNF signaling and leads to the development of depression-like behaviors in mice.
| ||
Cancer Med microRNA-196a-5p inhibits testicular germ cell tumor progression via NR6A1/E-cadherin axis.
| ||
J Clin Transl Hepatol Establishment of a Prognostic Model for Hepatocellular Carcinoma Based on Bioinformatics and the Role of NR6A1 in the Progression of HCC |
