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验证数据展示

  • IHC staining of human lung cancer using 14588-1-AP

    IHC staining of human lung cancer using 14588-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14588-1-AP (NRARP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 14588-1-AP

    IHC staining of human lung cancer using 14588-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14588-1-AP (NRARP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 14588-1-AP

    IF Staining of A431 using 14588-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NRARP antibody (14588-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

NRARP Polyclonal antibody

NRARP Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 14588-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

Notch-regulated ankyrin repeat-containing protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14588-1-AP targets NRARP in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 NRARP fusion protein Ag6114 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NOTCH-regulated ankyrin repeat protein
别名 Notch-regulated ankyrin repeat-containing protein
计算分子量 12 kDa
观测分子量12 kDa
GenBank蛋白编号BC053618
基因名称 NRARP
Gene ID (NCBI) 441478
RRIDAB_3085438
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z6K4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NRARP, Notch-regulated ankyrin repeat-containing protein, acts as a downstream effector of Notch signaling, involved in angiogenesis acting downstream of Notch at branch points to regulate vascular density. NRARP is also involved in the regulation of liver cancer cells self-renewal (PMID: 25985737, PMID: 19154719). During somitogenesis, it is involved in maintenance of proper somite segmentation and proper numbers of somites and vertebrae.

实验方案

Product Specific Protocols
IHC protocol for NRARP antibody 14588-1-APDownload protocol
IF protocol for NRARP antibody 14588-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols