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NRARP Polyclonal antibody

NRARP Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14588-1-AP

Print datasheet

Synonyms

Notch-regulated ankyrin repeat-containing protein

验证数据展示

  • IHC staining of human lung cancer using 14588-1-AP

    IHC staining of human lung cancer using 14588-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14588-1-AP (NRARP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 14588-1-AP

    IHC staining of human lung cancer using 14588-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14588-1-AP (NRARP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 14588-1-AP

    IF Staining of A431 using 14588-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NRARP antibody (14588-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14588-1-AP targets NRARP in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen NRARP fusion protein Ag6114 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NOTCH-regulated ankyrin repeat protein
Synonyms Notch-regulated ankyrin repeat-containing protein
Calculated Molecular Weight 12 kDa
Observed Molecular Weight12 kDa
GenBank Accession NumberBC053618
Gene Symbol NRARP
Gene ID (NCBI) 441478
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z6K4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NRARP, Notch-regulated ankyrin repeat-containing protein, acts as a downstream effector of Notch signaling, involved in angiogenesis acting downstream of Notch at branch points to regulate vascular density. NRARP is also involved in the regulation of liver cancer cells self-renewal (PMID: 25985737, PMID: 19154719). During somitogenesis, it is involved in maintenance of proper somite segmentation and proper numbers of somites and vertebrae.

实验方案

Product Specific Protocols
IHC protocol for NRARP antibody 14588-1-APDownload protocol
IF protocol for NRARP antibody 14588-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols