NRAS-Specific Polyclonal antibody

NRAS-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18296-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat, mouse

应用

WB, IHC, ELISA

ALPS4, GTPase NRas, HRAS1, N ras, NRAS, NRAS1, NRAS-Specific, Transforming protein N Ras

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA431 cells, MCF-7 cells
Positive IHC detected inhuman colon tissue, human kidney tissue, human colon cancer tissue, rat colon tissue, mouse colon tissue, human malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NRAS Ready-To-Use IHC Kit. NRAS primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18296-1-AP targets NRAS-Specific in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, rat, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neuroblastoma RAS viral (v-ras) oncogene homolog
别名 ALPS4, GTPase NRas, HRAS1, N ras, NRAS, NRAS1, NRAS-Specific, Transforming protein N Ras
计算分子量 21 kDa
观测分子量21 kDa
GenBank蛋白编号BC005219
基因名称 NRAS
Gene ID (NCBI) 4893
RRIDAB_2918061
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP01111
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NRAS, also named as N-ras and NRAS1, is neuroblastoma RAS viral (v-ras) oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML). The antibody is specific to NRAS, can't recognize HRAS and KRAS.

实验方案

Product Specific Protocols
WB protocol for NRAS-Specific antibody 18296-1-APDownload protocol
IHC protocol for NRAS-Specific antibody 18296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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