• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

NRAS-Specific Polyclonal antibody

NRAS-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18296-1-AP

Print datasheet

Synonyms

ALPS4, GTPase NRas, HRAS1, N ras, NRAS, NRAS1, NRAS-Specific, Transforming protein N Ras

验证数据展示

  • WB analysis using 18296-1-AP

    WB analysis using 18296-1-AP

    Recombinant fusion protein Ag27444 (KRAS-2A), Ag21645(KRAS-2B), Ag29746 (NRAS), and Ag23265(HRAS) were subjected to SDS PAGE followed by western blot with 18296-1-AP (NRAS-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 18296-1-AP

    WB analysis using 18296-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18296-1-AP (NRAS-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 18296-1-AP

    IHC staining of human colon using 18296-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NRAS antibody included
  • IHC staining of human colon using 18296-1-AP

    IHC staining of human colon using 18296-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NRAS antibody included

经过测试的应用

Positive WB detected inA431 cells, MCF-7 cells
Positive IHC detected inhuman colon tissue, human kidney tissue, human colon cancer tissue, rat colon tissue, mouse colon tissue, human malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NRAS Ready-To-Use IHC Kit. NRAS primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18296-1-AP targets NRAS-Specific in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, rat, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neuroblastoma RAS viral (v-ras) oncogene homolog
Synonyms ALPS4, GTPase NRas, HRAS1, N ras, NRAS, NRAS1, NRAS-Specific, Transforming protein N Ras
Calculated Molecular Weight 21 kDa
Observed Molecular Weight21 kDa
GenBank Accession NumberBC005219
Gene Symbol NRAS
Gene ID (NCBI) 4893
RRIDAB_2918061
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01111
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NRAS, also named as N-ras and NRAS1, is neuroblastoma RAS viral (v-ras) oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML). The antibody is specific to NRAS, can't recognize HRAS and KRAS.

实验方案

Product Specific Protocols
WB protocol for NRAS-Specific antibody 18296-1-APDownload protocol
IHC protocol for NRAS-Specific antibody 18296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols