验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy NRBF2 Ready-To-Use IHC Kit. NRBF2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24858-1-AP targets NRBF2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | NRBF2 fusion protein Ag21403 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nuclear receptor binding factor 2 |
| 别名 | Nuclear receptor-binding factor 2, NRBF-2, NRBF 2, COPR2, COPR1 |
| 计算分子量 | 32 kDa |
| 观测分子量 | 35-37 kDa |
| GenBank蛋白编号 | BC001345 |
| 基因名称 | NRBF2 |
| Gene ID (NCBI) | 29982 |
| RRID | AB_2879759 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96F24 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NRBF2 also termed as Nuclear receptor-binding factor 2or COPR is a 287 amino acid protein, which Interacts with PPARA, PPARD and PPARG. NRBF2 as transcription activator may modulate transcriptional activation by target nuclear receptors. NRBF2 is expressed in keratinocytes, liver and placenta. NRBF2, which exists as two isoforms due to alternative splicing, is localized to both the nucleus and the cytoplasm.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NRBF2 antibody 24858-1-AP | Download protocol |
| IHC protocol for NRBF2 antibody 24858-1-AP | Download protocol |
| IF protocol for NRBF2 antibody 24858-1-AP | Download protocol |
| IP protocol for NRBF2 antibody 24858-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy The mammalian actin elongation factor ENAH/MENA contributes to autophagosome formation via its actin regulatory function | ||
J Neuroinflammation Autophagy protein NRBF2 attenuates endoplasmic reticulum stress-associated neuroinflammation and oxidative stress via promoting autophagosome maturation by interacting with Rab7 after SAH.
| ||
iScience NRBF2 regulates the chemoresistance of small cell lung cancer by interacting with the P62 protein in the autophagy process.
| ||
Lab Invest Nuclear receptor binding factor 2 (NRBF2) is required for learning and memory. | ||
Nat Struct Mol Biol Reduction of DHHC5-mediated beclin 1 S-palmitoylation underlies autophagy decline in aging |
