NRD1 Polyclonal antibody

NRD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15630-1-AP

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Synonyms

hNRD1, hNRD2, N arginine dibasic convertase, Nardilysin, NRD C, NRD convertase, NRD1



经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inmouse testis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15630-1-AP targets NRD1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen NRD1 fusion protein Ag7923 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nardilysin (N-arginine dibasic convertase)
Synonyms hNRD1, hNRD2, N arginine dibasic convertase, Nardilysin, NRD C, NRD convertase, NRD1
Calculated Molecular Weight 132 kDa
Observed Molecular Weight 135-140 kDa
GenBank Accession NumberBC008775
Gene Symbol NRD1
Gene ID (NCBI) 4898
RRIDAB_2154525
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43847
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NRD1(Nardilysin) is also named as NRD-C(N-arginine dibasic convertase). It is a zinc metalloendopeptidase of the M16 pitrilysin family that cleaves peptides at dibasic residues. The enzyme has also been found in neural tissues of mouse embryos indicating a role in neural development. Although nardilysin is primarily found in the cytosol, it has been reported that nardilysin is located on the cell surface acting as a receptor for heparinbinding EGF-like growth factor(PMID:15809022). It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for NRD1 antibody 15630-1-APDownload protocol
IHC protocol for NRD1 antibody 15630-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Diabetes

Nardilysin-dependent proteolysis of cell-associated VTCN1 (B7-H4) marks type 1 diabetes development.

Authors - Ilian A Radichev
mouseWB

J Immunol

Loss of Peripheral Protection in Pancreatic Islets by Proteolysis-Driven Impairment of VTCN1 (B7-H4) Presentation Is Associated with the Development of Autoimmune Diabetes.

Authors - Ilian A Radichev
humanIHC

J Clin Med

Serum Nardilysin as a Prognostic Biomarker in Pancreatic Ductal Adenocarcinoma.

Authors - Yongfeng Xu