验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, human lung tissue, HEK-293T cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12482-1-AP targets NRF1/nuclear respiratory factor 1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, chicken, zebrafish, goat, duck, ducks |
| 免疫原 | NRF1/nuclear respiratory factor 1 fusion protein Ag3172 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nuclear respiratory factor 1 |
| 别名 | NRF1, nuclear respiratory factor 1, NRF-1, NRF 1, Alpha-pal |
| 计算分子量 | 522 aa, 56 kDa |
| 观测分子量 | 67 kDa, 45 kDa |
| GenBank蛋白编号 | BC016925 |
| 基因名称 | NRF1 |
| Gene ID (NCBI) | 4899 |
| RRID | AB_2282876 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16656 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NRF1/nuclear respiratory factor 1 antibody 12482-1-AP | Download protocol |
| IHC protocol for NRF1/nuclear respiratory factor 1 antibody 12482-1-AP | Download protocol |
| IP protocol for NRF1/nuclear respiratory factor 1 antibody 12482-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Hazard Mater Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers | ||
Redox Biol Defective mitochondrial ISCs biogenesis switches on IRP1 to fine tune selective mitophagy. | ||
Cell Commun Signal Local GHR roles in regulation of mitochondrial function through mitochondrial biogenesis during myoblast differentiation | ||
Mater Today Bio Platelet-derived extracellular vesicles ameliorate intervertebral disc degeneration by alleviating mitochondrial dysfunction |
