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NRF1/nuclear respiratory factor 1 Monoclonal antibody, PBS Only

NRF1/nuclear respiratory factor 1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C2B8

Cat no : 66832-1-PBS

Print datasheet

Synonyms

NRF1, nuclear respiratory factor 1, NRF 1, Alpha-pal, Alpha palindromic-binding protein

验证数据展示

  • WB analysis using 66832-1-Ig (same clone as 66832-1-PBS)

    WB analysis using 66832-1-Ig (same clone as 66832-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66832-1-Ig (NRF1/nuclear respiratory factor 1) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66832-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66832-1-Ig (same clone as 66832-1-PBS)

    IHC staining of human colon cancer using 66832-1-Ig (same clone as 66832-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66832-1-Ig (NRF1/nuclear respiratory factor 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66832-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NRF1 antibody included
  • IF Staining of HepG2 using 66832-1-Ig (same clone as 66832-1-PBS)

    IF Staining of HepG2 using 66832-1-Ig (same clone as 66832-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NRF1/nuclear respiratory factor 1 antibody (66832-1-Ig, Clone: 3C2B8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66832-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66832-1-Ig (same clone as 66832-1-PBS)

    FC experiment of HepG2 using 66832-1-Ig (same clone as 66832-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human NRF1/nuclear respiratory factor 1 (66832-1-Ig, Clone:3C2B8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66832-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy NRF1 Ready-To-Use IHC Kit. NRF1 primary antibody included.

产品信息

66832-1-PBS targets NRF1/nuclear respiratory factor 1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NRF1/nuclear respiratory factor 1 fusion protein Ag28149 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name nuclear respiratory factor 1
Synonyms NRF1, nuclear respiratory factor 1, NRF 1, Alpha-pal, Alpha palindromic-binding protein
Calculated Molecular Weight 522 aa, 56 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC016925
Gene Symbol NRF1
Gene ID (NCBI) 4899
RRIDAB_2882175
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16656
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.