验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, A431 cells, mouse liver tissue, MCF-7 cells, A549 cells, rat brain tissue, K-562 cells, NIH/3T3 cells, mouse brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human cervical cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21542-1-AP targets GABPA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | GABPA fusion protein Ag16191 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | GA binding protein transcription factor, alpha subunit 60kDa |
| 别名 | NFT2, GABP subunit alpha, GA-binding protein alpha chain, GA binding protein alpha chain, E4TF1A |
| 计算分子量 | 454 aa, 51 kDa |
| 观测分子量 | 56-60 kDa |
| GenBank蛋白编号 | BC035031 |
| 基因名称 | GABPA |
| Gene ID (NCBI) | 2551 |
| RRID | AB_10858481 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q06546 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GA-binding protein alpha chain (GABP alpha subunit, GABPA, nuclear respiratory factor 2 subunit alpha, transcription factor E4TF1-60) is one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit.GABPA is a member of Ets family, binds to the Yap promoter and activates YAP transcription(23684612). Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GABPA antibody 21542-1-AP | Download protocol |
| IHC protocol for GABPA antibody 21542-1-AP | Download protocol |
| IF protocol for GABPA antibody 21542-1-AP | Download protocol |
| IP protocol for GABPA antibody 21542-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Hepatology High mobility group protein B1 controls liver cancer initiation through yes-associated protein -dependent aerobic glycolysis. | ||
Cell Rep The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.
| ||
Cardiovasc Res Sex differences in exercise-induced physiological myocardial hypertrophy are modulated by oestrogen receptor beta. | ||
Stem Cells MZF1 and GABP Cooperate with Sox2 in Regulating the Expression of YAP1 in Cancer Cells.
| ||
Biology (Basel) TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis. | ||
BMC Cancer GABPA predicts prognosis and inhibits metastasis of hepatocellular carcinoma.
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