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验证数据展示

  • WB analysis of HeLa using 66504-1-Ig (same clone as 66504-1-PBS)

    WB analysis of HeLa using 66504-1-Ig (same clone as 66504-1-PBS)

    Untreated and MG132 treated Hela cells were subjected to SDS PAGE followed by western blot with 66504-1-Ig (NRF2, NFE2L2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66504-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50316-2

    Cytometric bead array standard curve of MP50316-2

    Cytometric bead array standard curve of MP50316-2, NRF2, NFE2L2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66504-2-PBS. Detection antibody: 66504-1-PBS. Standard:Ag9469. Range: 1.563-100 ng/mL.

  • Featured Product
  • KD/KO验证

NRF2, NFE2L2 Monoclonal antibody, PBS Only (Detector)

NRF2, NFE2L2 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 66504-1-PBS

产品说明书

CloneNo. 1E9E3

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, Cytometric bead array, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NFE2L2/NRF2 Ready-To-Use IHC Kit. NFE2L2/NRF2 primary antibody included.

产品信息

66504-1-PBS targets NRF2, NFE2L2 as part of a matched antibody pair:

MP50316-2: 66504-2-PBS capture and 66504-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 NRF2, NFE2L2 fusion protein Ag9469 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 nuclear factor (erythroid-derived 2)-like 2
别名
计算分子量 605 aa, 68 kDa
观测分子量 110 kDa
GenBank蛋白编号BC011558
基因名称 NRF2
Gene ID (NCBI) 4780
RRIDAB_2881867
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16236
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the actually observed molecular weight of Nrf2 is about 70kDa and 95-110 kDa. (PMID: 22703241).