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验证数据展示

  • IF Staining of MCF-7 using 31043-1-AP

    IF Staining of MCF-7 using 31043-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NRG1, isoform Alpha antibody (31043-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 31043-1-AP

    WB analysis using 31043-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31043-1-AP (NRG1, isoform Alpha antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

NRG1, isoform Alpha Polyclonal antibody

NRG1, isoform Alpha Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31043-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse, Rat

应用

WB, IF/ICC, ELISA

别名

ARIA, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRG1, HRGA, NDF, Neu differentiation factor, neuregulin 1, NRG1, NRG1, isoform Alpha, NRG1A, Pro NRG1, SMDF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, A549 cells, HeLa cells, MCF-7 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31043-1-AP targets NRG1, isoform Alpha in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 NRG1, isoform Alpha fusion protein Ag35114 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neuregulin 1
别名 ARIA, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRG1, HRGA, NDF, Neu differentiation factor, neuregulin 1, NRG1, NRG1, isoform Alpha, NRG1A, Pro NRG1, SMDF
计算分子量 70 kDa
观测分子量 70 kDa
GenBank蛋白编号BC007675
基因名称 NRG1
Gene ID (NCBI) 3084
RRIDAB_3669828
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ02297
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The immunogen of this antibody is against NRG1 isoform Alpha.

实验方案

Product Specific Protocols
WB protocol for NRG1, isoform Alpha antibody 31043-1-APDownload protocol
IF protocol for NRG1, isoform Alpha antibody 31043-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols