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验证数据展示

  • IF Staining of rat brain using 83251-2-RR

    IF Staining of rat brain using 83251-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NRG1, isoform Alpha antibody (83251-2-RR, Clone: 240067A6 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 83251-2-RR

    IF Staining of MCF-7 using 83251-2-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NRG1, isoform Alpha antibody (83251-2-RR, Clone: 240067A6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

NRG1, isoform Alpha Recombinant antibody

NRG1, isoform Alpha Uni-rAbTM Recombinant Antibody for IF/ICC, IF-P, ELISA
Cat No. 83251-2-RR

产品说明书

CloneNo. 240067A6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, IF-P, ELISA

别名

NRG1, NRG1A, Heregulin, Glial growth factor, GGF2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inrat brain tissue
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83251-2-RR targets NRG1, isoform Alpha in IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 NRG1, isoform Alpha fusion protein Ag35114 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 neuregulin 1
别名 NRG1, NRG1A, Heregulin, Glial growth factor, GGF2
计算分子量 70 kDa
观测分子量 70 kDa
GenBank蛋白编号BC007675
基因名称 NRG1
Gene ID (NCBI) 3084
RRIDAB_3670926
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ02297
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The immunogen of this antibody is against NRG1 isoform Alpha.

实验方案

Product Specific Protocols
IF protocol for NRG1, isoform Alpha antibody 83251-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols