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NRG1, isoform Alpha Recombinant antibody

NRG1, isoform Alpha Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240067A6

Cat no : 83251-2-RR

Print datasheet

Synonyms

NRG1, NRG1A, Heregulin, Glial growth factor, GGF2

验证数据展示

  • IF Staining of MCF-7 using 83251-2-RR

    IF Staining of MCF-7 using 83251-2-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NRG1, isoform Alpha antibody (83251-2-RR, Clone: 240067A6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83251-2-RR

    Affinity and Kinetic Characterization of 83251-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83251-2-RR against Human USP30 were performed. The affinity constant is 11.9 nM.

经过测试的应用

Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83251-2-RR targets NRG1, isoform Alpha in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen NRG1, isoform Alpha fusion protein Ag35114 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name neuregulin 1
Synonyms NRG1, NRG1A, Heregulin, Glial growth factor, GGF2
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC007675
Gene Symbol NRG1
Gene ID (NCBI) 3084
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ02297
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The immunogen of this antibody is against NRG1 isoform Alpha.

实验方案

Product Specific Protocols
IF protocol for NRG1, isoform Alpha antibody 83251-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols