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验证数据展示

  • WB analysis using 31147-1-AP

    WB analysis using 31147-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31147-1-AP (NRG1, isoform Beta1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 31147-1-AP

    IHC staining of mouse cerebellum using 31147-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31147-1-AP (NRG1, isoform Beta1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 31147-1-AP

    IHC staining of mouse cerebellum using 31147-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31147-1-AP (NRG1, isoform Beta1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 31147-1-AP

    IF Staining of MCF-7 using 31147-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NRG1, isoform Beta1 antibody (31147-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

NRG1, isoform Beta1 Polyclonal antibody

NRG1, isoform Beta1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31147-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse, Rat

应用

WB, IHC, IF/ICC, ELISA

别名

NRG1, isoform Beta1, NRG1, NRG1A, Pro NRG1, Pro-neuregulin-1, membrane-bound isoform, Pro-NRG1, NRG1 isoform SMDF, NRG1, isoform Alpha, NRG1, isoform SMDF, ARIA, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRG1, HRGA, NDF, Neu differentiation factor, neuregulin 1, Neuregulin 1 isoform SMDF, Neuregulin-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31147-1-AP targets NRG1, isoform Beta1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 NRG1, isoform Beta1 fusion protein Ag35188 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neuregulin 1
别名 NRG1, isoform Beta1, NRG1, NRG1A, Pro NRG1, Pro-neuregulin-1, membrane-bound isoform, Pro-NRG1, NRG1 isoform SMDF, NRG1, isoform Alpha, NRG1, isoform SMDF, ARIA, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRG1, HRGA, NDF, Neu differentiation factor, neuregulin 1, Neuregulin 1 isoform SMDF, Neuregulin-1
计算分子量 70 kDa
观测分子量 71 kDa
GenBank蛋白编号BC007675
基因名称 NRG1
Gene ID (NCBI) 3084
RRIDAB_3669870
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ02297
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The immunogen of this antibody is against NRG1 isoform Beta1.

实验方案

Product Specific Protocols
WB protocol for NRG1, isoform Beta1 antibody 31147-1-APDownload protocol
IHC protocol for NRG1, isoform Beta1 antibody 31147-1-APDownload protocol
IF protocol for NRG1, isoform Beta1 antibody 31147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols