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NRG1, isoform Beta1 Polyclonal antibody

NRG1, isoform Beta1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31147-1-AP

Print datasheet

Synonyms

NRG1, isoform Beta1, NRG1, NRG1A, Pro NRG1, Pro-neuregulin-1, membrane-bound isoform, Pro-NRG1, NRG1 isoform SMDF, NRG1, isoform Alpha, NRG1, isoform SMDF, ARIA, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRG1, HRGA, NDF, Neu differentiation factor, neuregulin 1, Neuregulin 1 isoform SMDF, Neuregulin-1

验证数据展示

  • WB analysis using 31147-1-AP

    WB analysis using 31147-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31147-1-AP (NRG1, isoform Beta1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 31147-1-AP

    IHC staining of mouse cerebellum using 31147-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31147-1-AP (NRG1, isoform Beta1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 31147-1-AP

    IHC staining of mouse cerebellum using 31147-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31147-1-AP (NRG1, isoform Beta1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 31147-1-AP

    IF Staining of MCF-7 using 31147-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NRG1, isoform Beta1 antibody (31147-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31147-1-AP targets NRG1, isoform Beta1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen NRG1, isoform Beta1 fusion protein Ag35188 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neuregulin 1
Synonyms NRG1, isoform Beta1, NRG1, NRG1A, Pro NRG1, Pro-neuregulin-1, membrane-bound isoform, Pro-NRG1, NRG1 isoform SMDF, NRG1, isoform Alpha, NRG1, isoform SMDF, ARIA, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRG1, HRGA, NDF, Neu differentiation factor, neuregulin 1, Neuregulin 1 isoform SMDF, Neuregulin-1
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC007675
Gene Symbol NRG1
Gene ID (NCBI) 3084
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02297
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The immunogen of this antibody is against NRG1 isoform Beta1.

实验方案

Product Specific Protocols
WB protocol for NRG1, isoform Beta1 antibody 31147-1-APDownload protocol
IHC protocol for NRG1, isoform Beta1 antibody 31147-1-APDownload protocol
IF protocol for NRG1, isoform Beta1 antibody 31147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols