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NRG4 Polyclonal antibody

NRG4 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 27292-1-AP

Print datasheet

Synonyms

HRG4, neuregulin 4, NRG 4, NRG4, Pro NRG4

验证数据展示

  • IHC staining of human prostate cancer using 27292-1-AP

    IHC staining of human prostate cancer using 27292-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27292-1-AP (NRG4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 27292-1-AP

    IHC staining of human prostate cancer using 27292-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27292-1-AP (NRG4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman prostate cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27292-1-AP targets NRG4 in IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human
Immunogen NRG4 fusion protein Ag26044 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neuregulin 4
Synonyms HRG4, neuregulin 4, NRG 4, NRG4, Pro NRG4
Calculated Molecular Weight 13 kDa
GenBank Accession NumberBC017568
Gene Symbol NRG4
Gene ID (NCBI) 145957
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WWG1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuregulin 4 (Nrg4), a member of the epidermal growth factor (EGF) family of extracellular ligands, is expressed in multiple organs with the highest expression levels in brown adipose tissue. Neuregulin 4 is a secreted adipokine recently identified as playing an important role in modulating systemic energy metabolism and in the development of metabolic disorders in rodent and human obesity, including type 2 diabetes and non-alcoholic fatty liver disease (NAFLD). Nrg4 attenuates hepatic lipogenic signaling and preserves glucose and lipid homeostasis in obesity.

实验方案

Product Specific Protocols
WB protocol for NRG4 antibody 27292-1-APDownload protocol
IHC protocol for NRG4 antibody 27292-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols