Neurogranin Polyclonal antibody
Neurogranin Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
| IF | See 1 publications below |
产品信息
10440-1-AP targets Neurogranin in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | Neurogranin fusion protein Ag0676 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | neurogranin (protein kinase C substrate, RC3) |
| Synonyms | hng, Neurogranin, Ng, NRGN, P17, RC3 |
| Calculated Molecular Weight | 7.6 kDa |
| Observed Molecular Weight | 15 kDa |
| GenBank Accession Number | BC002835 |
| Gene Symbol | Neurogranin |
| Gene ID (NCBI) | 4900 |
| RRID | AB_2251586 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92686 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Neurogranin (formerly designated p17, also known as Ng, RC3 and BICKS) belongs to the neurogranin family. It is a neuron-specific substrate for protein kinase C (PKC). It is a postsynaptic protein that is highly enriched in brain, with restricted expression in the cortex, striatum, hippocampus, thalamus, hypothalamus and olfactory bulb nuclei. Neurogranin binds calmodulin at low levels of calcium, thereby regulating calmodulin-dependent nitric oxide synthase. Neurogranin acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. It binds to calmodulin in the absence of calcium.The observed molecular weight of human NRGN by Western blotting is about 15-17 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Neurogranin antibody 10440-1-AP | Download protocol |
| IHC protocol for Neurogranin antibody 10440-1-AP | Download protocol |
| IP protocol for Neurogranin antibody 10440-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cells Specificity and Redundancy of Profilin 1 and 2 Function in Brain Development and Neuronal Structure. | ||
Elife An ancient germ cell-specific RNA-binding protein protects the germline from cryptic splice site poisoning. |