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Host
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Species Reactivity
0
Species Reactivity
0
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0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 83593-5-RR (same clone as 83593-5-PBS)

    WB analysis using 83593-5-RR (same clone as 83593-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83593-5-RR (Neurogranin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83593-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 83593-5-RR (same clone as 83593-5-PBS)

    IHC staining of mouse brain using 83593-5-RR (same clone as 83593-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83593-5-RR (Neurogranin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83593-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 83593-5-RR (same clone as 83593-5-PBS)

    IHC staining of mouse brain using 83593-5-RR (same clone as 83593-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83593-5-RR (Neurogranin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83593-5-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 83593-5-RR (same clone as 83593-5-PBS)

    FC experiment of A549 using 83593-5-RR (same clone as 83593-5-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Neurogranin Recombinant antibody (83593-5-RR, Clone:240526B9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83593-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83593-5-RR

    Affinity and Kinetic Characterization of 83593-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83593-5-RR against Human NRGN were performed. The affinity constant is 0.304 nM.

Neurogranin Recombinant antibody, PBS Only

Neurogranin Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83593-5-PBS

产品说明书

CloneNo. 240526B9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), ELISA

别名

NRGN, P17, Ng, NEUG(55-78), hng

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83593-5-PBS targets Neurogranin in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Neurogranin fusion protein Ag0676 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 neurogranin (protein kinase C substrate, RC3)
别名 NRGN, P17, Ng, NEUG(55-78), hng
计算分子量 7.6 kDa
观测分子量 15-17 kDa
GenBank蛋白编号BC002835
基因名称 Neurogranin
Gene ID (NCBI) 4900
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ92686
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Neurogranin (formerly designated p17, also known as Ng, RC3 and BICKS) belongs to the neurogranin family. It is a neuron-specific substrate for protein kinase C (PKC). It is a postsynaptic protein that is highly enriched in brain, with restricted expression in the cortex, striatum, hippocampus, thalamus, hypothalamus and olfactory bulb nuclei. Neurogranin binds calmodulin at low levels of calcium, thereby regulating calmodulin-dependent nitric oxide synthase. Neurogranin acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. It binds to calmodulin in the absence of calcium.