验证数据展示
经过测试的应用
| Positive IHC detected in | human retinoblastoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 2 publications below |
产品信息
17388-1-AP targets NRL in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | NRL fusion protein Ag11277 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | neural retina leucine zipper |
| 别名 | D14S46E, neural retina leucine zipper, NRL, NRL MAF, RP27 |
| 计算分子量 | 237 aa, 26 kDa |
| GenBank蛋白编号 | BC012395 |
| 基因名称 | NRL |
| Gene ID (NCBI) | 4901 |
| RRID | AB_2878400 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P54845 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NRL antibody 17388-1-AP | Download protocol |
| IHC protocol for NRL antibody 17388-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Human brain organoids assemble functionally integrated bilateral optic vesicles. | ||
JCI Insight All-in-one AAV-mediated Nrl gene inactivation rescues retinal degeneration in Pde6a mice | ||
Nat Protoc Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia |
