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NRL Polyclonal antibody

NRL Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17388-1-AP

Print datasheet

Synonyms

D14S46E, neural retina leucine zipper, NRL, NRL MAF, RP27

验证数据展示

  • IHC staining of human retinoblastoma using 17388-1-AP

    IHC staining of human retinoblastoma using 17388-1-AP

    Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 17388-1-AP (NRL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman retinoblastoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 2 publications below

产品信息

The immunogen of 17388-1-AP is NRL Fusion Protein expressed in E. coli.

Tested Applications IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen NRL fusion protein Ag11277 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neural retina leucine zipper
Synonyms D14S46E, neural retina leucine zipper, NRL, NRL MAF, RP27
Calculated Molecular Weight 237 aa, 26 kDa
GenBank Accession NumberBC012395
Gene Symbol NRL
Gene ID (NCBI) 4901
RRIDAB_2878400
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP54845
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NRL antibody 17388-1-APDownload protocol
IHC protocol for NRL antibody 17388-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell Stem Cell

Human brain organoids assemble functionally integrated bilateral optic vesicles.

Authors - Elke Gabriel
View Article
humanIF

Nat Protoc

Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia

Authors - Elke Gabriel
View Article