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验证数据展示

  • WB analysis of PC-3 using 23955-1-AP

    WB analysis of PC-3 using 23955-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 23955-1-AP (NSBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 23955-1-AP

    IHC staining of human testis using 23955-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:400 (under 40x lens).

  • IF Staining of HepG2 using 23955-1-AP

    IF Staining of HepG2 using 23955-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSBP1 antibody (23955-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

NSBP1 Polyclonal antibody

NSBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 23955-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Nucleosome-binding protein 1, NSBP 1, HMGN5, High mobility group nucleosome-binding domain-containing protein 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

23955-1-AP targets NSBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 NSBP1 fusion protein Ag20884 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nucleosomal binding protein 1
别名 Nucleosome-binding protein 1, NSBP 1, HMGN5, High mobility group nucleosome-binding domain-containing protein 5
计算分子量 282 aa, 32 kDa
观测分子量 55 kDa
GenBank蛋白编号BC005342
基因名称 NSBP1
Gene ID (NCBI) 79366
RRIDAB_2879374
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDP82970
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NSBP1, also named Nucleosome-binding protein 1 or HMGN5, is a 282 amino acid protein, which belongs to the HMGN family. NSBP1 localizes in the nucleus and is ubiquitously expressed. NSBP1 preferentially binds to euchromatin and modulates cellular transcription by counteracting linker histone-mediated chromatin compaction. Western blots shows the expression of NSBP1 as a 55kDa protein in human bladder cancer cells. (PMID: 21695596).

实验方案

Product Specific Protocols
WB protocol for NSBP1 antibody 23955-1-APDownload protocol
IHC protocol for NSBP1 antibody 23955-1-APDownload protocol
IF protocol for NSBP1 antibody 23955-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Eur Rev Med Pharmacol Sci

HMGN5 promotes invasion and migration of colorectal cancer through activating FGF/FGFR pathway.

Authors - G-J Zhu
View Article