验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27346-1-AP targets NSD1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | NSD1 fusion protein Ag26425 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nuclear receptor binding SET domain protein 1 |
| 别名 | ARA267, H3 K36 HMTase, H4 K20 HMTase, KMT3B, Lysine N methyltransferase 3B, NSD1, SOTOS, STO |
| 观测分子量 | 267-310 kDa |
| GenBank蛋白编号 | BC150628 |
| 基因名称 | NSD1 |
| Gene ID (NCBI) | 64324 |
| RRID | AB_3085948 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96L73 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NSD1 (nuclear receptor SET (su(var)3-9, enhancer-of-zeste,trithorax) domain containing protein-1), which is isolated and characterized in 2001, belongs to the NSD protein lysine methyltransferase (KMT) family. This family has three members, NSD1 (KMT3B), NSD2 (WHSC1/MMSET) and NSD3 (WHSC1L1), which could regulate the expression of target genes through methylation of lysine 36 on histone H3(H3K36). NSD1 could bind various promoter elements to regulate transcription via interactions with H3K36 methylation and RNA polymerase II.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NSD1 antibody 27346-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |