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NSD1 Polyclonal antibody

NSD1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27346-1-AP

Print datasheet

Synonyms

ARA267, H3 K36 HMTase, H4 K20 HMTase, KMT3B, Lysine N methyltransferase 3B, NSD1, SOTOS, STO

验证数据展示

  • WB analysis using 27346-1-AP

    WB analysis using 27346-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 27346-1-AP (NSD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27346-1-AP targets NSD1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen NSD1 fusion protein Ag26425 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear receptor binding SET domain protein 1
Synonyms ARA267, H3 K36 HMTase, H4 K20 HMTase, KMT3B, Lysine N methyltransferase 3B, NSD1, SOTOS, STO
Observed Molecular Weight267-310 kDa
GenBank Accession NumberBC150628
Gene Symbol NSD1
Gene ID (NCBI) 64324
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96L73
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NSD1 (nuclear receptor SET (su(var)3-9, enhancer-of-zeste,trithorax) domain containing protein-1), which is isolated and characterized in 2001, belongs to the NSD protein lysine methyltransferase (KMT) family. This family has three members, NSD1 (KMT3B), NSD2 (WHSC1/MMSET) and NSD3 (WHSC1L1), which could regulate the expression of target genes through methylation of lysine 36 on histone H3(H3K36). NSD1 could bind various promoter elements to regulate transcription via interactions with H3K36 methylation and RNA polymerase II.

实验方案

Product Specific Protocols
WB protocol for NSD1 antibody 27346-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols