验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human heart tissue, mouse brain tissue |
| Positive IHC detected in | human skin cancer tissue, human cervical cancer tissue, human colon tissue, human colon cancer tissue, human lung tissue, human lung cancer tissue, human ovary tissue, human ovary tumor tissue, human stomach tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3200 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 9 publications below |
| IHC | See 7 publications below |
产品信息
15111-1-AP targets NSDHL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | NSDHL fusion protein Ag7097 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NAD(P) dependent steroid dehydrogenase-like |
| 别名 | XAP104, Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating, SDR31E1, Protein H105e3, H105E3 |
| 计算分子量 | 42 kDa |
| 观测分子量 | 42 kDa, 40 kDa |
| GenBank蛋白编号 | BC000245 |
| 基因名称 | NSDHL |
| Gene ID (NCBI) | 50814 |
| RRID | AB_2155681 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15738 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NSDHL(Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating) encodes a sterol dehydrogenase or decarboxylase involved in the sequential removal of two C-4 methyl groups in post-squalene cholesterol biosynthesis. Mutations in this gene are associated with human CHILD syndrome (congenital hemidysplasia with ichthyosiform nevus and limb defects), an X-linked, male lethal disorder, as well as the mouse mutations bare patches and striated.(PMID: 14506130).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NSDHL antibody 15111-1-AP | Download protocol |
| IHC protocol for NSDHL antibody 15111-1-AP | Download protocol |
| IF protocol for NSDHL antibody 15111-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell Cholesterol Pathway Inhibition Induces TGF-β Signaling to Promote Basal Differentiation in Pancreatic Cancer.
| ||
Cancer Discov Targeting C4-demethylating genes in the cholesterol pathway sensitizes cancer cells to EGF receptor inhibitors via increased EGF receptor degradation. | ||
Clin Cancer Res Statins Synergize with Hedgehog Pathway Inhibitors for Treatment of Medulloblastoma.
| ||
Cell Rep Endogenous Sterol Metabolites Regulate Growth of EGFR/KRAS-Dependent Tumors via LXR. | ||
Cell Cycle Insm2 deficiency results in female infertility by disturbing steroid pathway and decreasing ovarian reserve in mice. | ||
