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NSF Polyclonal antibody

NSF Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 21172-1-AP

Print datasheet

Synonyms

NEM sensitive fusion protein, NSF, SKD2, Vesicle fusing ATPase, Vesicular fusion protein NSF

验证数据展示

  • WB analysis using 21172-1-AP

    WB analysis using 21172-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21172-1-AP (NSF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 21172-1-AP

    IP experiment of mouse brain using 21172-1-AP

    IP result of anti-NSF (IP:21172-1-AP, 4ug; Detection:21172-1-AP 1:300) with mouse brain tissue lysate 3000ug.

  • IHC staining of mouse brain using 21172-1-AP

    IHC staining of mouse brain using 21172-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21172-1-AP (NSF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • IHC staining of mouse brain using 21172-1-AP

    IHC staining of mouse brain using 21172-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21172-1-AP (NSF Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

21172-1-AP targets NSF in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen NSF fusion protein Ag15422 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name N-ethylmaleimide-sensitive factor
Synonyms NEM sensitive fusion protein, NSF, SKD2, Vesicle fusing ATPase, Vesicular fusion protein NSF
Calculated Molecular Weight 744 aa, 83 kDa
Observed Molecular Weight 83 kDa
GenBank Accession NumberBC030613
Gene Symbol NSF
Gene ID (NCBI) 4905
RRIDAB_2878823
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP46459
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The N-ethylmaleimide-sensitive fusion protein (NSF) is a 70-83 kDa cytosol protein that is expressed in all cell types. NSF functions as an ATPase and it is a part of the 20S membrane fusion apparatus with the vesicular SNAP receptor (v-SNARE) synaptobrevin, and the target SNAREs (t-SNARE) syntaxin and SNAP-25. With the hydrolysis of ATP by NSF, the released energy may set-up or drive membrane fusion processes. NSF is required for general membrane transport and trafficking.

实验方案

Product Specific Protocols
WB protocol for NSF antibody 21172-1-APDownload protocol
IHC protocol for NSF antibody 21172-1-APDownload protocol
IP protocol for NSF antibody 21172-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell Death Differ

Transglutaminase 2 crosslinks zona pellucida glycoprotein 3 to prevent polyspermy.

Authors - Zhaokang Cui
View Article