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验证数据展示

  • WB analysis using 28837-1-AP

    WB analysis using 28837-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28837-1-AP (NSF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 28837-1-AP

    IHC staining of mouse brain using 28837-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28837-1-AP (NSF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 28837-1-AP

    IHC staining of mouse brain using 28837-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28837-1-AP (NSF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NSF Polyclonal antibody

NSF Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28837-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

NEM sensitive fusion protein, NSF, SKD2, Vesicle fusing ATPase, Vesicular fusion protein NSF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28837-1-AP targets NSF in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 NSF fusion protein Ag30229 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 N-ethylmaleimide-sensitive factor
别名 NEM sensitive fusion protein, NSF, SKD2, Vesicle fusing ATPase, Vesicular fusion protein NSF
计算分子量 744 aa, 83 kDa
观测分子量70-83 kDa
GenBank蛋白编号BC030613
基因名称 NSF
Gene ID (NCBI) 4905
RRIDAB_2918208
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP46459
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The N-ethylmaleimide-sensitive fusion protein (NSF) is a 70-83 kDa cytosol protein that is expressed in all cell types. NSF functions as an ATPase and it is a part of the 20S membrane fusion apparatus with the vesicular SNAP receptor (v-SNARE) synaptobrevin, and the target SNAREs (t-SNARE) syntaxin and SNAP-25. With the hydrolysis of ATP by NSF, the released energy may set-up or drive membrane fusion processes. NSF is required for general membrane transport and trafficking.

实验方案

Product Specific Protocols
WB protocol for NSF antibody 28837-1-APDownload protocol
IHC protocol for NSF antibody 28837-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols