验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HEK-293 cells, mouse liver tissue |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human lung cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15620-1-AP targets NSFL1C in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IP, IHC, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse |
免疫原 | NSFL1C fusion protein Ag8040 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | NSFL1 (p97) cofactor (p47) |
别名 | NSFL1 (p97) cofactor (p47), NSFL1 cofactor p47, NSFL1C, p47, p97 cofactor p47, UBX1, UBXD10, UBXN2C |
计算分子量 | 41 kDa |
观测分子量 | 47 kDa, 41 kDa |
GenBank蛋白编号 | BC002801 |
基因名称 | NSFL1C |
Gene ID (NCBI) | 55968 |
RRID | AB_2878158 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9UNZ2 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for NSFL1C antibody 15620-1-AP | Download protocol |
IHC protocol for NSFL1C antibody 15620-1-AP | Download protocol |
IP protocol for NSFL1C antibody 15620-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Proc Natl Acad Sci U S A Altered cofactor regulation with disease-associated p97/VCP mutations. | ||
Cell Chem Biol Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase. | ||
J Biol Chem Pathogenic mutations in the Valosin-containing protein/p97(VCP) N-domain inhibit the SUMOylation of VCP and lead to impaired stress response. | ||
Evid Based Complement Alternat Med Shenxian-Shengmai Oral Liquid Improves Sinoatrial Node Dysfunction through the PKC/NOX-2 Signaling Pathway. |