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NSFL1C Polyclonal antibody

NSFL1C Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 15620-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA and More (1)

NSFL1 (p97) cofactor (p47), NSFL1 cofactor p47, NSFL1C, p47, p97 cofactor p47, UBX1, UBXD10, UBXN2C

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, mouse liver tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15620-1-AP targets NSFL1C in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 NSFL1C fusion protein Ag8040 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NSFL1 (p97) cofactor (p47)
别名 NSFL1 (p97) cofactor (p47), NSFL1 cofactor p47, NSFL1C, p47, p97 cofactor p47, UBX1, UBXD10, UBXN2C
计算分子量 41 kDa
观测分子量 47 kDa, 41 kDa
GenBank蛋白编号BC002801
基因名称 NSFL1C
Gene ID (NCBI) 55968
RRIDAB_2878158
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UNZ2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NSFL1C antibody 15620-1-APDownload protocol
IHC protocol for NSFL1C antibody 15620-1-APDownload protocol
IP protocol for NSFL1C antibody 15620-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

Altered cofactor regulation with disease-associated p97/VCP mutations.

Authors - Xiaoyi Zhang
humanWB

Cell Chem Biol

Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase.

Authors - Xiaojun Wu
humanWB

J Biol Chem

Pathogenic mutations in the Valosin-containing protein/p97(VCP) N-domain inhibit the SUMOylation of VCP and lead to impaired stress response.

Authors - Tao Wang
humanWB,IF

J Cell Sci

The p97-UBXN1 complex regulates aggresome formation.

Authors - Sirisha Mukkavalli
  • KD Validated
mouseWB

Evid Based Complement Alternat Med

Shenxian-Shengmai Oral Liquid Improves Sinoatrial Node Dysfunction through the PKC/NOX-2 Signaling Pathway.

Authors - Heng Zhang
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